Posts Tagged ‘two-photon’

Dynamic Regulation of Eve Stripe 2 Expression Reveals Transcriptional Bursts in Living Drosophila Embryos

Jacques P. Bothma, Hernan G. Garcia, Emilia Esposito, Gavin Schlissel, Thomas Gregor, Michael S. Levine. PNAS 111 (29): 10598–10603 (2014).
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Quantitative imaging of transcription in living Drosophila embryos links polymerase activity to patterning

Hernan G. Garcia, Mikhail Tikhonov, Albert Lin and Thomas Gregor. Current Biology 23, 2140–2145 (2013).
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Quantifying the Bicoid morphogen gradient in living fly embryos

Alexander H. Morrison, Martin Scheeler Julien O. Dubuis and Thomas GregorCold Spring Harb Protoc. 2012(4): 398-406 (2012).
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About the tools we use

For starters, some images:

Laser Scanning Two-Photon Microscope

Laser Scanning Two-Photon Microscope

Detail of a custom built Two-Photon Microscope

Illustration of custom-built two-photon microscope with trans- and epi-detection (also note embryo in special holder).

Illustration of custom-built two-photon microscope with trans- and epi-detection (also note embryo in special holder).